Hello, I was trying to use mutibamsummary tool to generate the read coverage for 8 filtered Bam files(using SAMTOOLS, Filter SAM or BAM, output SAM or BAM ). These 8 filtered Bam files came from the ChIP seq data, which were mapped to the same genome(using Bowtie). But got error saying "No common chromosomes found". I was wondering why this happened? Also, is there any tutorials about detecting the methylation enrichment on histone by using ChIP seq analysis? Thank you very much, Ren
Did you sort the BAM datasets first? Was that successful? https://biostar.usegalaxy.org/p/27855
If there is still a problem, and you can reproduce the problem at Galaxy Main https://usegalaxy.org, please send in a bug report. A tool problem is a possibility, and if present, we'll want to fix it. Please leave all inputs and outputs undeleted and include a link to this post in the comments. https://galaxyproject.org/support/tool-error/
I don't know of a Galaxy tutorial that covers that exact analysis. I linked in the current tutorials in the prior Q&A. If you are interested in helping to develop one, the Galaxy Training Network (GTN) would welcome contributions.
- About the GTN: https://galaxyproject.org/community/#galaxy-training-network
- Gitter channel: https://gitter.im/Galaxy-Training-Network/Lobby
- Upcoming Training workshop on 5/21/18: https://galaxyproject.org/galaxy-updates/2018-05/#upcoming-events
Thanks! Jen, Galaxy team