Hello, I was trying to use mutibamsummary tool to generate the read coverage for 8 filtered Bam files(using SAMTOOLS, Filter SAM or BAM, output SAM or BAM ). These 8 filtered Bam files came from the ChIP seq data, which were mapped to the same genome(using Bowtie). But got error saying "No common chromosomes found". I was wondering why this happened? Also, is there any tutorials about detecting the methylation enrichment on histone by using ChIP seq analysis? Thank you very much, Ren
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Question: multi bam summary
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lnaren • 10 wrote:
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modified 6 months ago
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Jennifer Hillman Jackson ♦ 25k
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6 months ago by
lnaren • 10
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Jennifer Hillman Jackson ♦ 25k wrote:
Hello,
Did you sort the BAM datasets first? Was that successful? https://biostar.usegalaxy.org/p/27855
If there is still a problem, and you can reproduce the problem at Galaxy Main https://usegalaxy.org, please send in a bug report. A tool problem is a possibility, and if present, we'll want to fix it. Please leave all inputs and outputs undeleted and include a link to this post in the comments. https://galaxyproject.org/support/tool-error/
I don't know of a Galaxy tutorial that covers that exact analysis. I linked in the current tutorials in the prior Q&A. If you are interested in helping to develop one, the Galaxy Training Network (GTN) would welcome contributions.
- About the GTN: https://galaxyproject.org/community/#galaxy-training-network
- Gitter channel: https://gitter.im/Galaxy-Training-Network/Lobby
- Upcoming Training workshop on 5/21/18: https://galaxyproject.org/galaxy-updates/2018-05/#upcoming-events
Thanks! Jen, Galaxy team
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