Question: Changing Parameters In Tophat
gravatar for Colaneri, Alejandro Cesar
5.5 years ago by
I want to run tophat with the parameter "--no-novel-juncs genome" Where do I find the options to add this parameter? Dr. Alejandro Colaneri Departments of Biology University of North Carolina at Chapel Hill 310 Coker Hall, CB# 3280 120 South Road Chapel Hill, NC 27599-3280 Tel: 919-962-2273 fax: 919- 962-1625
rna-seq tophat • 914 views
ADD COMMENTlink modified 5.5 years ago by Jennifer Hillman Jackson25k • written 5.5 years ago by Colaneri, Alejandro Cesar80
gravatar for Jennifer Hillman Jackson
5.5 years ago by
United States
Jennifer Hillman Jackson25k wrote:
Hello, On the Tophat tool form, if you scroll to the very bottom, the list of implemented option is listed. In general, the order will be the same as the options on the form. * Tophat parameter list* This is a list of implemented Tophat options: -G/--GTF [GTF 2.2 file] Supply TopHat with a list of gene model annotations. TopHat will use the exon records in this file to build a set of known splice junctions for each gene, and will attempt to align reads to these junctions even if they would not normally be covered by the initial mapping. -j/--raw-juncs [juncs file] Supply TopHat with a list of raw junctions. Junctions are specified one per line, in a tab-delimited format. Records look like: [chrom] [left] [right] [+/-], left and right are zero-based coordinates, and specify the last character of the left sequenced to be spliced to the first character of the right sequence, inclusive. -no-novel-juncs Only look for junctions indicated in the supplied GFF file. (ignored without -G) To set these, change " TopHat settings to use:" to be "Full parameter list" and the form will expand, showing in the full view all of the options. For these three, the labels are: Use Gene Annotation Model: Use Raw Junctions: Only look for supplied junctions: Hopefully this helps, Jen Galaxy team -- Jennifer Hillman-Jackson Galaxy Support and Training
ADD COMMENTlink written 5.5 years ago by Jennifer Hillman Jackson25k
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