Question: Which "Library Type" Should I Use For Single-End Reads
gravatar for Du, Jianguang
5.6 years ago by
Du, Jianguang110
Du, Jianguang110 wrote:
Hi All, I have a very basic question about parameters for running TopHat. I have datasets of single-end reads. These datasets were generated with Illumina Genome Analyzer IIx. Which "Library Type" should I choose to run Tophat? Thanks. Best, Jianguang
rna-seq tophat • 1.4k views
ADD COMMENTlink modified 5.6 years ago by Jennifer Hillman Jackson25k • written 5.6 years ago by Du, Jianguang110
gravatar for Jennifer Hillman Jackson
5.6 years ago by
United States
Jennifer Hillman Jackson25k wrote:
Hello Jianguang, This depends on how the library prep was done (which protocol was followed). Some preserve strand and some do not. If you did not prepare the data yourself, then the data source should be able to provide you with this information. Hopefully this helps, Jen Galaxy team -- Jennifer Hillman-Jackson Galaxy Support and Training
ADD COMMENTlink written 5.6 years ago by Jennifer Hillman Jackson25k
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