Question: Which "Library Type" Should I Use For Single-End Reads
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gravatar for Du, Jianguang
5.6 years ago by
Du, Jianguang • 110
Du, Jianguang • 110 wrote:
Hi All, I have a very basic question about parameters for running TopHat. I have datasets of single-end reads. These datasets were generated with Illumina Genome Analyzer IIx. Which "Library Type" should I choose to run Tophat? Thanks. Best, Jianguang
rna-seq tophat • 1.4k views
ADD COMMENT • link • modified 5.6 years ago by Jennifer Hillman Jackson ♦ 25k • written 5.6 years ago by Du, Jianguang • 110
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gravatar for Jennifer Hillman Jackson
5.6 years ago by
United States
Jennifer Hillman Jackson ♦ 25k wrote:
Hello Jianguang, This depends on how the library prep was done (which protocol was followed). Some preserve strand and some do not. If you did not prepare the data yourself, then the data source should be able to provide you with this information. Hopefully this helps, Jen Galaxy team -- Jennifer Hillman-Jackson Galaxy Support and Training http://galaxyproject.org
ADD COMMENT • link written 5.6 years ago by Jennifer Hillman Jackson ♦ 25k
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