Question: Fwd: Galaxy: Rna-Seq Analysis Problems
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James Taylor ♦ 470 wrote:
Roberta, I'm traveling right now so I'm forwarding your message to our
help list. Thanks.
Date: Tue, Sep 11, 2012 at 5:19 AM
Subject: Re: Galaxy: RNA-seq analysis problems
To: James Taylor <james@jamestaylor.org>
Hello James,
sorry to bother you again, but I've one more question for you. I know
that most existing methodologies to analyze RNA-seq data, have a
strong dependency on sequencing depth for their differential
expression calls and that this results might have a considerable
number of false positives. Unfortunately, 1 out of 3 biological
replicates of a set of my samples have a much bigger seq depth with
respect to the other two samples. Do the programs in the Galaxy NGS:
RNA Analysis section take into account this problem and normalize it?
Thank you in advance for you help,
Roberta Galletti.
Glad to hear it! Thanks!
James,
I managed to make it work. Thank you for your help.
Roberta.
--
Roberta Galletti, PhD
Laboratoire de Reproduction et Développement des Plantes
Ecole Normale Supérieure de Lyon, UMR 5667
46, allée d'Italie
69364 LYON cedex 07
FRANCE
e-mail 1: roberta.galletti@ens-lyon.fr
e-mail 2: ro.galletti@tiscalinet.it
Skype contact: roberta1977
...A lab is just another place to play....
From 'Dancing naked in the mind field'
Kary B. Mullis, Nobel Prize in Chemistry 1993.
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Jennifer Hillman Jackson ♦ 25k
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James Taylor ♦ 470
