Question: Should I Run Fastq Groomer?
0
gravatar for Du, Jianguang
5.8 years ago by
Du, Jianguang370
Du, Jianguang370 wrote:
Dear All, I have some FASTQ datasets in phred 33 offset, and I have already assinged them Fastqsanger format. Do I need to run FASTQ Groomer on these datasets before I check the data quality by "Fastqc: Fastqc QC" and "FASTQ Trimmer by column" to remove bad nucleotides at 3' end of reads? Should I select "Sanger" as "Input FASTQ quality scores type:" if I need to run Groomer? Thanks. Jianguang Du
• 850 views
ADD COMMENTlink modified 5.8 years ago by Jennifer Hillman Jackson25k • written 5.8 years ago by Du, Jianguang370
0
gravatar for Jennifer Hillman Jackson
5.8 years ago by
United States
Jennifer Hillman Jackson25k wrote:
Hello Jianguang, Given this information, you will not need to run FASTQ Groomer, but simply proceed with Fastqc and FASTQ Trimmer. Best, Jen Galaxy team -- Jennifer Jackson http://galaxyproject.org
ADD COMMENTlink written 5.8 years ago by Jennifer Hillman Jackson25k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 125 users visited in the last hour