Question: Gene Ontology Help
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Nicole Lynn Jacobs • 10 wrote:
To Whom it May Concern:
My name is Nicole McDaniels. I am a Ph.D. candidate at Syracuse
University. I am attempting to use RNAseq to look at changes in the
transcriptome of zebrafish embryos upon morpholino injection. I have
been using Galaxy Main to manage my data thus far. Recently, I
completed the cufflinks/cuffcompare/cuffdiff analysis. I now have
cuffdiff files in the tabular format. I would like to assess the gene
ontologies of the differentially expressed transcripts, but I'm having
trouble figuring out how to transfer over to GO Galaxy and then what
exactly I should be doing in GO Galaxy.
Is there a different program or server that would be better/easier for
the gene ontology analysis than GO Galaxy? I've also looked at the
GOseq R package, but again I'm not sure about the tabular file format
or how to change it to a more appropriate format. Any help you can
give me with this would be greatly appreciated. I thank you in advance
for your time.
All the best,
Nicole McDaniels
Ph.D Candidate
Syracuse University
Department of Biology
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modified 3.5 years ago
by
ajmaninisha • 30
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written
7.0 years ago by
Nicole Lynn Jacobs • 10
