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Question: Mapping Tags
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gravatar for Soetkin Versteyhe
7.0 years ago by
Soetkin Versteyhe • 30
Soetkin Versteyhe • 30 wrote:
Dear all, I would like to map (e.g. with Bowtie) collapsed sequences (tags) instead of individual sequence reads. Does anyone know if this is possible in Galaxy? Thank you in advance. Best regards, Soetkin Versteyhe, PhD PostDoc University of Copenhagen Faculty of Health Sciences The Novo Nordisk Foundation Center for Basic Metabolic Research Integrative Physiology Blegdamsvej 3B 2200 Křbenhavn N Denmark PHONE +45 35337116 soetkin.versteyhe@sund.ku.dk http://sund.ku.dk http://metabol.ku.dk<http: metabol.ku.dk=""/> [cid:image001.gif@01CCA385.44DE02C0]
alignment bowtie • 504 views
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modified 7.0 years ago by Jennifer Hillman Jackson ♦ 25k • written 7.0 years ago by Soetkin Versteyhe • 30
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gravatar for Jennifer Hillman Jackson
7.0 years ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:
Hello Soetkin, Is your concern that the sequences will be in FASTA format (without quality scores) instead of FASTQ format? If so, the Galaxy tool "NGS: QC and manipulation -> Combine FASTA and QUAL into FASTQ" can create placeholder quality values in a FASTQ file appropriate for use with NGS mapping tools. Hopefully this helps. If you would like further help, please explain the issue in more detail and consider sending a small sample of the data pasted into the email (10 or so entries) if that is relevant. For reference: http://wiki.g2.bx.psu.edu/Support#Public_mailing_list_Q_.26_A_discussi ons Thanks for using Galaxy! Jen Galaxy team -- Jennifer Jackson http://usegalaxy.org http://galaxyproject.org/wiki/Support
ADD COMMENT • link written 7.0 years ago by Jennifer Hillman Jackson ♦ 25k
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