Question: Mapping Tags
gravatar for Soetkin Versteyhe
7.0 years ago by
Soetkin Versteyhe30 wrote:
Dear all, I would like to map (e.g. with Bowtie) collapsed sequences (tags) instead of individual sequence reads. Does anyone know if this is possible in Galaxy? Thank you in advance. Best regards, Soetkin Versteyhe, PhD PostDoc University of Copenhagen Faculty of Health Sciences The Novo Nordisk Foundation Center for Basic Metabolic Research Integrative Physiology Blegdamsvej 3B 2200 Křbenhavn N Denmark PHONE +45 35337116<http:""/> [cid:image001.gif@01CCA385.44DE02C0]
alignment bowtie • 504 views
ADD COMMENTlink modified 7.0 years ago by Jennifer Hillman Jackson25k • written 7.0 years ago by Soetkin Versteyhe30
gravatar for Jennifer Hillman Jackson
7.0 years ago by
United States
Jennifer Hillman Jackson25k wrote:
Hello Soetkin, Is your concern that the sequences will be in FASTA format (without quality scores) instead of FASTQ format? If so, the Galaxy tool "NGS: QC and manipulation -> Combine FASTA and QUAL into FASTQ" can create placeholder quality values in a FASTQ file appropriate for use with NGS mapping tools. Hopefully this helps. If you would like further help, please explain the issue in more detail and consider sending a small sample of the data pasted into the email (10 or so entries) if that is relevant. For reference: ons Thanks for using Galaxy! Jen Galaxy team -- Jennifer Jackson
ADD COMMENTlink written 7.0 years ago by Jennifer Hillman Jackson25k
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