I have multiple single-cell RNA-seq samples for which I have a generated a featureCount output for each sample. Now I want to do PCA, but it requires a single dataset input. Please, how do I combine all my featureCount output files into a format that the PCA will accept?
Most PCA tools would require a matrix as input and not a collection of single-column tables. This is actually easy in Galaxy if you use the Column Join tool, assuming your featureCount tables are all in the same format (which they should be).
After that you should have a matrix (tabular) that you can feed into your PCA