Question: Problems loading/using custom genome dataset for Hisat analysis
gravatar for p_solorzano77
5 months ago by
p_solorzano770 wrote:


I am trying to load from ENA the genome dataset of Carnegiea gigantea into HiSAT2 analysis for annotation of a Hylocereus transcriptome. I uploaded the reference genome PRJNA318822 into Galaxy but it is still not available as a choosable genome from my history profile. The only issue I noticed is that the file format is fast.q.gz. Could that be the issue? I am using the latest HISAT2 version.

Best regards,


build genome galaxy custom • 174 views
ADD COMMENTlink modified 5 months ago by Jennifer Hillman Jackson25k • written 5 months ago by p_solorzano770
gravatar for Jennifer Hillman Jackson
5 months ago by
United States
Jennifer Hillman Jackson25k wrote:


The data needs to be loaded in fasta format and assigned to that datatype.

Please see this support FAQ for full details:

Thanks! Jen, Galaxy team

ADD COMMENTlink written 5 months ago by Jennifer Hillman Jackson25k
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