Latest
Open
RNA-Seq
ChIP-Seq
SNP
Assembly
Forum
Planet
All »
Home
Log In
Question: HISAT2-reference genome not available in a local -- data must be installed first
0
gravatar for c2c12
6 months ago by
c2c12 • 10
NYC
c2c12 • 10 wrote:

Hi everyone, When I'm trying to use in my local galaxy HISAT2 for mapping the reads I have an information that reference genome is not available. I'm interested in grch37. I already downloaded one from Ensembl but I'd like to figure out how to use the one built in HISAT2. I'd be grateful for help with this issue. Z.
hisat local genome galaxy indexes • 242 views
ADD COMMENT • link •
modified 6 months ago by Jennifer Hillman Jackson ♦ 25k • written 6 months ago by c2c12 • 10
0
gravatar for Jennifer Hillman Jackson
6 months ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

The hg19 genome is indexed for HISAT2 at Galaxy Main https://usegalaxy.org. This is the UCSC version of GRCh37.

When using a local, you need to install the genome and indexes for tools. Genome data is not included in the distribution.

Best options -- all require that you are an administrator:

The final option is to use the genome as a custom reference genome. This uses much more resource than pre-computed indexes and you might run into memory problems, it depends on your configuration. It works well for small genomes (at any server) but even Galaxy Main can produce memory-related tool failures when using very large custom genomes like Human. https://galaxyproject.org/learn/custom-genomes/

Thanks, Jen, Galaxy team
ADD COMMENT • link written 6 months ago by Jennifer Hillman Jackson ♦ 25k
Please log in to add an answer.

Similar posts • Search »

Content
Help
Access
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 172 users visited in the last hour