Question: How to add and use a custom reference genome
gravatar for wy412
6 months ago by
wy4120 wrote:

Dear Sir or Madam,

May I know how to add some other genome information for custemerized analysis on some other plant genome reference, such as Sesamum indicum. There is genome information in NCBI database. How to upload to Galaxy and do RNA-seq analysis?

Hope you can teach me to solve this problem.

Thanks and best regards,

Your sincerely, Yi

fasta build genome custom rna-seq • 266 views
ADD COMMENTlink modified 6 months ago by Jennifer Hillman Jackson25k • written 6 months ago by wy4120
gravatar for Jennifer Hillman Jackson
6 months ago by
United States
Jennifer Hillman Jackson25k wrote:


Download the fasta version of the genome and upload it to Galaxy. Then use it as a Custom reference genome with tools. Getting the associated reference annotation GTF would also be a good idea, as it is commonly utilized or required by RNA-seq tools.

Use FTP to load any data over 2 GB in size up to Galaxy.

Support FAQs:

Galaxy Tutorials, including those for RNA-seq:

Thanks, Jen, Galaxy team

ADD COMMENTlink written 6 months ago by Jennifer Hillman Jackson25k
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