I have aligned reads to Brassica juncea genome 1.5 using HISAT2. Now i want to perform PCA analysis to check my replicates. For this i am sorting BAM files by chromosomal coordinates but I am getting error; SORT An error occurred setting the metadata for this dataset Set it manually or retry auto-detection
SortSAM Fatal error: Exit code 127 () /galaxy-repl/main/jobdir/018/286/18286619/tool_script.sh: line 9: picard: command not found
multiBAMSummary Fatal error: Exit code 1 () [bwHdrRead] There was an error while reading in the header! Traceback (most recent call last): File "/galaxy/main/deps/_conda/envs/mulled-v1-0fa290085c742a3ffee6a142d1fce47c178e1c289ff6f0c66023b506f3377842/bin/multiBamSummary", line 11, in <module> main(args) File "/galaxy/main/deps/_conda/envs/mulled-v1-0fa290085c742a3ffee6a142d1fce47c178e1c289ff6f0c66023b506f3377842/lib/python2.7/site-packages/deeptools/multiBamSummary.py", line 224, in main num_reads_per_bin = c.run(allArgs=args) File "/galaxy/main/deps/_conda/envs/mulled-v1-0fa290085c742a3ffee6a142d1fce47c178e1c289ff6f0c66023b506f3377842/lib/python2.7/site-packages/deeptools/countReadsPerBin.py", line 244, in run y = pyBigWig.open(x) RuntimeError: Received an error during file opening!