Question: Adding reference genome to Galaxy
0
gravatar for tylertibetli
13 days ago by
tylertibetli0 wrote:

Hi, I want to add reference genome(hg19) from UCSC main table to my Galaxy account,how can I do that?I want to realign my reads and when I try to use GATK realigner,it asks reference genome.

alignment galaxy • 55 views
ADD COMMENTlink modified 13 days ago • written 13 days ago by tylertibetli0
1
gravatar for Jennifer Hillman Jackson
13 days ago by
United States
Jennifer Hillman Jackson23k wrote:

Hello,

The GATK tools hosted at https://usegalaxy.org are from a very old version (1.4) and have been deprecated for some time now. These are natively indexed for hg_g1k_v37 (1000 Genomes) and not hg19 (UCSC). Using hg19 from the history as a custom reference genome will cause several to fail for exceeding memory resources. We strongly recommend that you avoid using these tools.

Instead, please see the variant analysis tutorials here that include the most current tools and methods:

Thanks! Jen, Galaxy team

ADD COMMENTlink written 13 days ago by Jennifer Hillman Jackson23k
0
gravatar for tylertibetli
13 days ago by
tylertibetli0 wrote:

Hi Jen,

Thank you for your assistance.

ADD COMMENTlink written 13 days ago by tylertibetli0
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