Question: FileZilla FTP application is not able to retrieve galaxy directory listing
gravatar for aritrancbs.research
14 months ago by
aritrancbs.research10 wrote:
Status:         Resolving address of
Status:         Connecting to
Status:         Connection established, waiting for welcome message...
Status:         Initializing TLS...
Status:         Verifying certificate...
Status:         TLS connection established.
Status:         Logged in
Status:         Retrieving directory listing...
Command:    PWD
Response:   257 "/" is the current directory
Command:    TYPE I
Response:   200 Type set to I
Command:    PASV
Response:   227 Entering Passive Mode (129,114,60,56,118,102).
Command:    MLSD
Error:          Connection timed out after 20 seconds of inactivity

This is what i am getting after repeated attempts of tryin to connect to galaxy via Filezilla. I have been following the video tutorials of galaxy for uploading via FTP, but nothing seems to work. Any idea what i should do?

Error:          Failed to retrieve directory listing
Status:         Disconnected from server
chip-seq • 970 views
ADD COMMENTlink modified 14 months ago by aritra.misra120 • written 14 months ago by aritrancbs.research10
gravatar for Nate Coraor
14 months ago by
Nate Coraor3.2k
United States
Nate Coraor3.2k wrote:

Is there a firewall at your site that might be interfering? It seems like something - either on the host or on that particular network - is blocking connections.

In order to make a passive FTP connection, the FTP client opens a connection to a control channel: port 21 in the case of Explicit FTPS or non-secure connections, port 990 in the case of Implicit FTPS. After authenticating, the client attempts to switch to passive mode - the PASV command. This instructs the FTP server on our side to create a random port (in our case, this will be between 30000 and 31000) for your client to connect to for data transfers. FileZilla attempts to get a directory listing from the data port with MLSD but times out when attempting to connect to the data port.

There could be multiple reasons for this, however, I suspect the reason is that some firewalls inspect traffic to (remote) port 21 and watch for the response to the PASV command so they can allow outbound traffic from the client to the data port returned by PASV (in this case IP: port: 118*256+102=30310). Since the connection becomes encrypted after issuing AUTH TLS, the firewall cannot read the PASV response and thus connections to the data port fail.

As a first step, please try connecting with Implicit FTPS documentation and see if you have the same result.

Also, do you get this message upon initially connecting (i.e. you are unable to connect at all) or after successfully connecting, when trying to upload?

ADD COMMENTlink written 14 months ago by Nate Coraor3.2k
gravatar for aritra.misra12
14 months ago by
aritra.misra120 wrote:

While connecting through Filezilla, i made sure that the firewall is disabled. Moreover i think i am not able to connect to galaxy at all because i am getting this message at the initial stage. I have tried with implicite as well as explicite FTPS and both have given the same result.

Is there any other way of uploading above 2GB data onto galaxy?

ADD COMMENTlink written 14 months ago by aritra.misra120

I realize this is a pain, but if you are able to split your files, there is a concatenate function in the main galaxy server which can align flat files head-to-tail. Hope you get the FTP to work though!

ADD REPLYlink written 14 months ago by renae.geier40

What's the error message when connecting with implicit FTPS?

ADD REPLYlink written 14 months ago by Nate Coraor3.2k
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