Hi Biostars community, I attempting to run some initial QC and manipulation tools on some rnaseq data (illumina 1.8). I have my trimmed data from Trim Galore!, however I am unable to pair the trimmed data with FASTQ joiner. Whenever I take the two paired outputs (in fastqsanger format) from Trim Galore and use them in FASTQ joiner, the program will run, but returns a message states that 0% of reads were paired, no matter the "old" or "new" header option is selected.
"There were 22817078 known sequence reads not utilized. Joined 0 of 22817078 read pairs (0.00%)."
My Trim Galore data is in the following format: @D00472:148:CB1Y1ANXX:2:2201:1059:2134 1:N:0:ATCACG CTCAGGCATAGGTCACCAGCTTTCGGGTCGTTTGCCAACTGCTCAACCTCTGCACAGTCACAAGTGACACGCACAGGGCCGTGGTGCGCTGCACTCCG + BBBBBBFBF/</bbffff>
Am I missing something?
I have used this workflow in the past with success, but now it does not seem to work. I have noticed that this error popped up about 5 years ago, however the work around solution calls on a tool that is not available anymore. Any help/suggestions would be greatly appreciated.