Question: Metagenomics workflow / tutorial using paired-end sequencing (HiSeq)
gravatar for Darshan Baral
19 months ago by
Darshan Baral0 wrote:

I came across this galaxy workflow that was originally used with 454 sequences.

I was wondering if it would also work with Hi-seq data (HiSeq 2500 2x125 PE)

ADD COMMENTlink modified 19 months ago by Jennifer Hillman Jackson25k • written 19 months ago by Darshan Baral0
gravatar for Jennifer Hillman Jackson
19 months ago by
United States
Jennifer Hillman Jackson25k wrote:


The most current metagenomics tutorial with a Galaxy workflow is here:

The Windshield Splatter publication's workflow was designed for single end sequencing. It is also a bit outdated, so you will not find several of the tools at the Galaxy Main public server at anymore (if you plan to do the analysis there).

However, all of the original tools are in the Tool Shed. These are for use in a local or cloud Galaxy. Also, histories, datasets, and/or workflows can be exported/imported between Galaxy servers. The entire analysis is reproducible.

Thanks! Jen, Galaxy team

ADD COMMENTlink written 19 months ago by Jennifer Hillman Jackson25k
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