Question: How do I convert numerals to nucleotides for RNA-Seq mapping?
gravatar for turton
8 months ago by
United States
turton0 wrote:

The FASTQ reads I imported from EBI SRA (SRR064843) appear to be numeric (0-3) rather than AGCT. How do I convert by trimmed reads to nucleotide format so that I can do read mapping? Thanks!

rna-seq • 146 views
ADD COMMENTlink modified 8 months ago by Jennifer Hillman Jackson23k • written 8 months ago by turton0
gravatar for Jennifer Hillman Jackson
8 months ago by
United States
Jennifer Hillman Jackson23k wrote:


The tool Convert SOLiD output to fastq can be used (instructions on the tool form) on data retrieved using Get Data > EBI SRA from the link under the header "FASTQ files (Galaxy)". This places the fastq.gz file directly into your history. It can be used as is or click on the pencil icon and convert the format to uncompressed fastq.

Help pages

Thanks, Jen, Galaxy team

ADD COMMENTlink written 8 months ago by Jennifer Hillman Jackson23k

Thank you! :-) I'll try that

ADD REPLYlink written 7 months ago by turton0
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 83 users visited in the last hour