I am new at using Cufflinks. I was wondering if someone could help me figure what "Average Fragment Length" and "Fragment Length Standard Deviation" are. I was reading somewhere that the average fragment length can be determined by substracting the length of the adapters (122bp for illumina) from the average lenght obtained after bioanalyzer analysis of my libraries (300bp).
I don't know how to obtain the standard deviation though.
Any help would be much appreciated