Question: Reference genome can it be partial?
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gravatar for RACHAMPION
13 months ago by
RACHAMPION10
RACHAMPION10 wrote:

I'm doing an RNA-seq on wheat to get differential expression of wild type vs a mutant. I'm only looking at a few specific genes. The wheat genome has not been fully sequenced, and for this problem it does not need to be. NCBI lists transcriptomes only. Can I use a program (e.g. Tuxedo suite) that requires a reference (in this case, the transcriptome) to do the differential expression? I would prefer the reference genome approach because it is simpler than deNovo assembly.

Thanks

ADD COMMENTlink modified 13 months ago by shengweima0 • written 13 months ago by RACHAMPION10
0
gravatar for Jennifer Hillman Jackson
13 months ago by
United States
Jennifer Hillman Jackson24k wrote:

Hello,

Yes, these tools can be used with a transcriptome instead of a genome.

Use the Custom Reference genome feature with tools. It doesn't matter if the CG is partial. A CG is simply a fasta data in your history cleaned up with the tool NormalizeFasta using the options to wrap at 80 bases and to trim the title line at the first whitespace.

Thanks, Jen, Galaxy team

ADD COMMENTlink written 13 months ago by Jennifer Hillman Jackson24k
0
gravatar for shengweima
13 months ago by
shengweima0
shengweima0 wrote:

The wheat reference genome v1.0 has released. https://wheat-urgi.versailles.inra.fr/Seq-Repository/Assemblies I have used this reference in my rna_seq data.

ADD COMMENTlink written 13 months ago by shengweima0
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