Question: Re: Cufflinks Merging More Than One Transcript On Bacterial Genomes
6.8 years ago by
Jeremy Goecks • 2.2k
Jeremy Goecks • 2.2k wrote:
Noa, I'm not sure that folks on this list have much experience with bacterial transcriptome analysis. You might try seqanswers.com or try emailing the Tophat/Cufflinks authors directly: firstname.lastname@example.org If you find something interesting in another place, please feel free to share with the Galaxy community. Reference genome or reference gene annotation? Using a genome to correct for bias should not change the assembled transcripts, only their expression levels. You can use a reference gene annotation either as ground truth or as a guide; using the reference as ground truth ensures that Cufflinks will only assemble transcripts defined in the annotation. Good luck, J.
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