Hello all, Our lab currently has a large amount of bisulfite sequencing data that a collaborator of mine is looking at. He has methylation sequencing between two conditions. I was wondering whether I could use this same sequencing data to look at genomic variations/mutations (i.e. NHEJ, SNPs, Frame Shift, etc...) between the two samples. I understand how bisulfite sequencing works and what it is used for, but just want to know if there is a tool for this—i.e. something that converts that converted uracils/thymine back into the original cytosine. From there I could proceed from normal!
Thoughts? Thank you all.
Worst comes to worst we will have to repeat with normal WG DNA seq