Hi, I'm new in managing DNA sequences and I'm looking for help. I have fastq and vcf files from the sequencing of my samples. The plant species on which I work (Lagenaria siceraria) has no reference genome. What I want to do is to extract SNPs flanking sequences and make a blast in Plant RefSeq for determining the putative functions of my SNPs. All the posts Ive read are related to species that have model organism. Then, could someone help me please?