Question: combining FASTQ files from 3 lanes
gravatar for Gabi
2.4 years ago by
Gabi0 wrote:


I have data from multiple samples that were multiplexed and split across 3 lanes. These were demultiplexed by the sequencing facility, but I'm wondering how I can use galaxy to now merge the 3 FASTQ files that I have for each sample (ie. those that have the same barcode)?


rna-seq multiplex galaxy • 1.6k views
ADD COMMENTlink modified 2.4 years ago by Jennifer Hillman Jackson25k • written 2.4 years ago by Gabi0
gravatar for Jennifer Hillman Jackson
2.4 years ago by
United States
Jennifer Hillman Jackson25k wrote:


Would the tool Text Manipulation: Concatenate work for you? There are other tools in the group NGS: QC and manipulation that will combine sequences together (merge first two, then merge that result with the third - in order), but if you just need to merge the files head to tail, then the first tool will do that.

Thanks, Jen, Galaxy team

ADD COMMENTlink written 2.4 years ago by Jennifer Hillman Jackson25k
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