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Question: Why I can't use some of the tools
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gravatar for chenchen7
2.5 years ago by
chenchen7 • 0
chenchen7 • 0 wrote:

I have data from Illumina, as part1,fastq and part 2.fastq. First thing is that I want to join them together. The thing is when I wanted to use FASTQ joiner, it showed "No fastqsanger or fastqcssanger dataset available. Then, I turned to use "Pear Paired-End read merger", and it showed the part1.fastq as well as part2.fastq. Another example is that when I followed the Galaxy 101, I followed the protocol till "2.0. Joining exons with SNPs" step. I couldn't continue because the "Join" tool showed "No interval dataset available". Do you guys have the similar situation? How could I fix it? I will appreciate if I can get any feedbacks!
pear fastq joiner • 703 views
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modified 2.5 years ago by yena.oh • 70 • written 2.5 years ago by chenchen7 • 0
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gravatar for yena.oh
2.5 years ago by
yena.oh • 70
Canada
yena.oh • 70 wrote:

Hi,

For FASTQ joiner to work, your fastq files need to have its quality format changed to a fastqsanger format. This is done using the FASTQ Groomer tool. Run Groomer on your fastq files, then try doing running FASTQ joiner.

Hope this helps!

Yena
ADD COMMENT • link written 2.5 years ago by yena.oh • 70
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