Question: Why I can't use some of the tools
gravatar for chenchen7
2.5 years ago by
chenchen70 wrote:

I have data from Illumina, as part1,fastq and part 2.fastq. First thing is that I want to join them together. The thing is when I wanted to use FASTQ joiner, it showed "No fastqsanger or fastqcssanger dataset available. Then, I turned to use "Pear Paired-End read merger", and it showed the part1.fastq as well as part2.fastq. Another example is that when I followed the Galaxy 101, I followed the protocol till "2.0. Joining exons with SNPs" step. I couldn't continue because the "Join" tool showed "No interval dataset available". Do you guys have the similar situation? How could I fix it? I will appreciate if I can get any feedbacks!

pear fastq joiner • 703 views
ADD COMMENTlink modified 2.5 years ago by yena.oh70 • written 2.5 years ago by chenchen70
gravatar for yena.oh
2.5 years ago by
yena.oh70 wrote:


For FASTQ joiner to work, your fastq files need to have its quality format changed to a fastqsanger format. This is done using the FASTQ Groomer tool. Run Groomer on your fastq files, then try doing running FASTQ joiner.

Hope this helps!


ADD COMMENTlink written 2.5 years ago by yena.oh70
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