Question: Quality cut-off value
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gravatar for m_seifi
3.0 years ago by
m_seifi • 0
m_seifi • 0 wrote:

why should we set the Quality cut-off value to 20?

galaxy • 503 views
ADD COMMENT • link • modified 3.0 years ago by Jennifer Hillman Jackson ♦ 25k • written 3.0 years ago by m_seifi • 0
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gravatar for Jennifer Hillman Jackson
3.0 years ago by
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

A Sanger Phred+33 Quality Score of 20 means that there is 1% probability of base calling errors. That is often considered good enough, but you can decide. 

There is a table at the head of this wiki page that shows the breakdown, plus much more: Phred_quality_score

Best, Jen, Galaxy team

 

ADD COMMENT • link written 3.0 years ago by Jennifer Hillman Jackson ♦ 25k
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