Dear Galaxy Support:
My name is Wei-Ming Yu. I am a postdoctoral fellow from Department of Neurobiology at Harvard Medical School.
I am planing to analyze some RNA-Seq data using Galaxy in Amazon Web Service.
I followed the online document (https://wiki.galaxyproject.org/CloudMan/AWS/GettingStarted) to set up Galaxy in AWS and I have some questions regarding the setup using AWS.
Fisrt, is there a more updated instruction of how to set up AWS for Galaxy? The online one is a bit outdated as Amazon updates its AWS interface.
Second, for creating the security group, I can't change VPC to No VPC. It already assigned a default VPC-ID (vpc-2e83784a. Should I leave it as it is?
Finally, I am not sure the instance that I configured has enough resources for my project. I only signed up a Free Tier EC2 account and it only allows me to choose t1.micro as the instance type. I have 8 RNS-Seq samples (from mouse tissues) and each has thirty million pair-end reads. After unzipping the data, the fastq files are already 143GB. Would you suggest me to pay to upgrade to a better AWS service so that I won't wait indefinitely for processing my data. I am willing to pay for the better AWS service but would like your recommendation of how many resources should I obtain (instance type, vCPUs, storage)?
Thank you very much for your help! I really appreciate it.