Question: SAMtools Merge BAM files hangs
1
gravatar for kr81
3.1 years ago by
kr8110
Australia
kr8110 wrote:

Hi all,

I am using TopHat to map RNA-seq reads. My study species is a non-model organism, so I created a custom genome build in order to do this. The first time I tried to map, I got an error message (reference sequence has more than 2^32-1 characters), so I split the genome in two, created two builds and mapped the reads to each build separately. I now have two accepted_hits BAM files that I want to join before performing further analyses. I tried using SAMTools > Merge BAM Files (Galaxy GVL-QLD mirror) but the job runs for a week without being completed. I think it might be related to this issue https://github.com/samtools/samtools/issues/260 , as the reference genome contains over 1 million contigs, but I'm not sure. Can anyone suggest a work around? I would prefer to continue using the Galaxy GUI rather than the command line version of SAMtools if possible.

Thanks

 

galaxy samtools bam • 1.1k views
ADD COMMENTlink modified 3.1 years ago by Jennifer Hillman Jackson25k • written 3.1 years ago by kr8110
0
gravatar for Jennifer Hillman Jackson
3.1 years ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

The mapped datasets are based on a different reference genome (the two halves) so cannot be used in the same analysis. Much current bioinformatics computation is based on data positions relative to a common reference genome (or transcriptome, exome, etc). Without that, there is little that can be done between different datasets (only calculations that do not involve positional information).

Section 2.8 of the Galaxy support wiki explains alternatives for working with data/jobs that exceed the compute resources at http://usegalaxy.org:
https://wiki.galaxyproject.org/Support

Best, Jen, Galaxy team

ADD COMMENTlink written 3.1 years ago by Jennifer Hillman Jackson25k
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