I have downloaded my ChIP-seq data which I aligned to the human genome using Bowtie for Illumina on Galaxy, and have installed MACS on my computer (4Gb RAM) and gotten it running in CMD/DOS. Unfortunately my data files appear too large to run locally (25Gb [90 000 000 lines] input control, 30Gb [120 000 000 lines] ChIP). When I run MACS14 it returns:
Traceback (most recent call last):
File "macs14.py", line 366, in ,module.
File "macs14.py", liine 60, in main
(treat, control) = load_tag_files_options (options)
File "macs14.py", line 355, in load_tag_files_options
ttsize = tp.size()
File "C:\Python27\Lib\site-packages\MACS14\Parser.py", line 759, in tsize for x in rage(nc):
Is there any way for me to run this analysis (peak finding) on my computer? I can gain access to a 12Gb RAM computer, I just need to move my data files. Would that work, or do I need to look at running the analysis on a server? I don't have access to a server so I think I am stuck at this point.
Thanks for your help.