I am having difficulty figuring out the Tophat tool on Galaxy. I was trying to use the Tophat tool to map fastq files that I uploaded from EBI SRA. I am unable to run the Tophat, on it, since Tophat does not appear to be recognizing the file. I have tried to run Tophat on another fastq file from the RNA-Seq exercise of Galaxy:
This appears to be running fine. As far as I can tell, the only difference between the two files is that the fastq file from the exercise is formatted as fastqsanger, while I cannot see that on the file I uploaded. How can I work around this? Do I need to convert my files into a different format? If so, how can I do this?
Thanks for all your help and advice.
Edit: I am editing to include that I am using the usegalaxy.org site.