Question: An application that retrieves the read mapping results of a specific gene
0
gravatar for jchen015
3.8 years ago by
jchen01580
Singapore
jchen01580 wrote:

Hi guys

May I know where can i reference more about writing an application that is able to retrieve the read mapping of a specific gene, given the gene ID or the name of the gene.

I am planning to write it in python anyway...

Regards,

Julius

development application • 1.1k views
ADD COMMENTlink modified 3.8 years ago • written 3.8 years ago by jchen01580
1
gravatar for Bjoern Gruening
3.8 years ago by
Bjoern Gruening5.1k
Germany
Bjoern Gruening5.1k wrote:

Do you want to have the read-count for a specific gene?

ADD COMMENTlink written 3.8 years ago by Bjoern Gruening5.1k

Hey Bjoern

Yes I do. Do you have any article which i could review? or better if there is any source available?

Regards,

Julius

ADD REPLYlink written 3.8 years ago by jchen01580
2

Not really, but have a look at HTSeq-count. This tool is part of the ToolShed and can be installed into any Galaxy instance.

ADD REPLYlink written 3.8 years ago by Bjoern Gruening5.1k

Thanks for the advice Bhoern, however I am being tasked to develop an application by leveraging Galaxy's API. So was wondering which areas can i explore into..

ADD REPLYlink written 3.8 years ago by jchen01580
1

A good start to read about Galaxy's API is the following documenation: http://galaxy-central.readthedocs.org/en/latest/lib/galaxy.webapps.galaxy.api.html

However I am not sure how you plan on using Galaxy's API without being interested in the tools that will perform the analysis steps. Galaxy itself is an engine to run tools. Without the tools you can't do much.

ADD REPLYlink written 3.8 years ago by Martin Čech ♦♦ 4.9k

Thanks Martin

Is there currently a script that could retrieve the read mapping results of a specific gene, with given gene ID or gene name?

Regards,

Julius

ADD REPLYlink written 3.8 years ago by jchen01580

Hello, The solution here is most likely a Workflow that utilizes the tool that Bjoern mentions. Create the Workflow on your server (once the tool is loaded, or you wrap/load your own tool that perform the read->transcript mapping), add in the other tools/steps needed (input reference data, mapping transcript to gene names/IDs from the source you want to use, etc.). Then that Workflow can be executed through the API. Thanks, Jen, Galaxy team

ADD REPLYlink written 3.8 years ago by Jennifer Hillman Jackson25k
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