I've done Tophat with my sequencing reads and I'm trying to do Cufflinks. However, i keep getting this error sequence,
cufflinks -q --no-update-check -I 300000 -F 0.100000 -j 0.150000 -p 8 -N -b ref.fa
Error running cufflinks.
return code = 1
cufflinks -q --no-update-check -I 300000 -F 0.100000 -j 0.150000 -p 8 -N -b ref.fa /galaxy-repl/mai"
AND "Required metadata values are missing. Some of these values may not be editable by the user. Selecting "Auto-detect" will attempt to fix these values."
Does anyone know what is going on? At first I thought it might be my Reference sequence, but then the reads was mapped successfully using that sequence in the Tophat step.
Does anyone know how to solve the problem?