Similar posts • Search »

• Re: Quality Based Trimming
  Thanks for your reply Daniel. That's right... I did not even think about using the boxplot tool t...
• FastQ sanger sequences -ends quality trimming
  Dear all , I'm very naive regarding bioinformatic tools. I need to trim my sanger sequences to re...
• Trimmomatic Operation Error
  Hi, I try to trim my sequence with trimmomatic. But I cannot understand why my options are not ta...
• illumina PE quality control - NGS: QC & Manipulation
  Hi, I have a PE illumina miseq data set (separate forward-R1 & reverse-R2) of a WGS of a par...
• FASTQ trimmer trimmed 1 nt too much of sequence but right amount of quality
  Hi,   I added two fastq files (I had just changed the datatype to fastqcsanger) to FASTQ trimme...
• Question
  Hi Is it possible to trim a variable number of a specific nucleotide from the 3' ends of fastq R...
• Clipping adapter from 3' end of only 1 of 2 paired end reads
  Hi all, I cropped barcodes a long while back in a lab where I had CLC Genomics - now that I'm in...
• Pre-Processing Of Illumina Rna-Seq Paired End Data
  Hello, I have Illumina 76bp paired end data for a zebrafish RNA-seq experiment and am basically ...
• How Much Can I Trimm My Reads
  Dear All, I am analysing RNA-seq datasets for the differential splicing events between cell type...
• Analysing paired end RNAseq data with poor quality score
  Hello, I'm new in NGS and i'm currently working on RNA-seq. I had 6G of illumina reads and proce...
• unable to run tophat on trim galore processed reads
  dear sir i have run tophat on reads trimmed by trim galore with reference to hg19. i am getting r...
• Trimming And Thinning Of Paired-End Reads
  Hi galaxy users, I've been experiencing some problems trimming the adapters and filtering by qua...
• Bowtie on Galaxy - -v or -n ?
  Good morning, I'm using Galaxy with Bowtie for Illumina to map smallRNA sequencing (illumina) on ...
• FASTQC for RNA-Seq data in Galaxy
  Hello, I have 3 paired end fastaq file and when i loaded to Galaxy through EBI-SRA, it shows XX...
• Problem running Clustal 2.1 Multiple Sequences Alignment - an instance of 'std::bad_alloc'
  Dear All, I've been trying to analyze reads from a short transcript. The data I have was obtaine...
• Re: Quality Trimming Of Sequences Before Rna-Seq
  Dear Noa, I also feel the same. So I think it would be better to use these three steps remove s...
• 3' Adapter Trimming Using Fastx-Toolkit Clipper
  Hi all, I am analyzing miRNA sequencing now. My data is 51bp, single -ended and ~5 M reads. I wan...
• RNAseq data to be processed in two ways: (i) mapping to de novo Trinity-based transcriptome and (ii) mapping a relatively new genome
  Hello all, I am new to RNAseq data and learning this process step by step, so I have a few quest...