Question: Glitch In .Wig Visualization In Trackster?
5.7 years ago by
Michael Axtell • 60
Michael Axtell • 60 wrote:
Hi everyone. I'm having an issue with a wiggle file. I'm using Trackster on the public-main instance of Galaxy, with a custom genome build. My wiggle file fails to be shown. When added to the visualization using the 'add tracks' dialog, I see the usual hatched gray lines with the message "processing data, this may take some time". But then after a few minutes the track just goes to hatched gray lines with no messages, and the intensities are never displayed, nor is any error message (or any message at all, just stuck with the hatched gray lines). The .wig file has been extensively validated to conform to UCSC spec. In addition, the same file displays data just fine when loaded into Broad's IGV. So I'm confident it is formatted correctly. The custom genome is not a great one .. scaffolds not pseudomolecules, and there are many thousands of scaffolds in the assembly (scaffold N50 is 1.3M at scaffold 111 out of ~2,100 scaffolds; total length ~480M). If I slice my problematic wiggle file to only keep sub-sections of the data, sometimes it works. I tested a number of such sub-slices, and some worked and some didn't, as below (the numbers refer to scaffold numbers in my custom genome): 1-50 : worked 1-100 : worked 1-200 : worked 1-300 : failed 1-400 : failed 1-500 : failed 100-250 : worked 200-300 : worked 300-400 : worked 500-600 : worked doesn't like wig files that exceed a certain number of chromosomes/scaffolds? Or some sort of data overload issue? Some other information: This custom genome build works fine on trackster to visualize several other datasets in gff, gff3, and bed format. In addition, the problem wiggle file is not so large .. the full file is only ~48M. It is a fixedStep file with span and step both equal to 100, and the data are relatively sparse. If anyone has a clue, let me know .. thanks! -- Michael J. Axtell, Ph.D. Associate Professor Dept. of Biology Penn State University http://axtell-lab-psu.weebly.com
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