To see the tools available to work with GFF and GTF datafiles, please
see the tool group "Filter and Sort". Galaxy does not have a GFF->GFF3
converter - and I do not know of one. There is a GTF->GFF3 converter
the Tool Shed (details below).
First, you will want to make sure that you actually have GFF and not
(GFF is a much older format).
Next, GFF/GTF/GFF3 data can vary quite a bit, even those files that
the official format specifications. Also, not all would contain the
needed by another if a conversion were to be done (additional files
would be required or a different set of related starting files would
needed). Still, there are some tools available to convert GTF->GFF3.
example, you could try a local install (http://getgalaxy.org
) with the
repository from the Tool Shed named fml_gff3togtf containing a
A search with the term "convert GFF to GFF3" will bring up some
discussion at several bio chat sites about the issues with doing this,
along with other options and an online tool to do the same. How any of
these will function with your particular data and the final use (RNA-
pipeline?) is difficult to predict. Much depends on the inputs.
The best choice is to locate a file that has been already created or
that can be extracted from a public database using the tools that they
provide. Once you have a file in the appropriate format, the next step
is to make certain that the file contains the data the tools needs to
perform the calculations you are most interested in. The tool
documentation (link to manual) will explain expected inputs vs the
output. For GTF/GFF3 data, these data are often in the 9th field.
Conversion can be tricky, but you will likely find a method. The
for gene annotation' portion of your question is more complicated to
address and will likely take some testing on your part to see what
and what doesn't (to produce the expected results), but hopefully this
gives you some ideas about how to start.