Question: Reference Genome Format For Tophat
0
gravatar for i b
6.3 years ago by
i b330
i b330 wrote:
Dear all, I managed to upload to Galaxy a genome of interest in .fasta format from NCIB website. However, Galaxy does not recognize it as input to run Tophat... Wht format has to be to be used as referece genome for tophat?and how can i convert it? Any suggestion? thanks, ib
galaxy • 2.4k views
ADD COMMENTlink modified 6.3 years ago by Jennifer Hillman Jackson25k • written 6.3 years ago by i b330
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gravatar for Jennifer Hillman Jackson
6.3 years ago by
United States
Jennifer Hillman Jackson25k wrote:
Hello Irene, This wiki has the formatting details, including a screencast for genome prep, and many tips for correcting format problems that use Galaxy's tools whenever possible: http://wiki.g2.bx.psu.edu/Learn/CustomGenomes#Screencasts_.26_Tutorial s watch "Custom Genome Prep" http://wiki.g2.bx.psu.edu/Learn/CustomGenomes#Troubleshooting That said, it many simply be that the "datatype" is not actually set to be "fasta" for the dataset? Do this by clicking on the pencil icon to reach the "Edit Attributes" form, set this manually, and save. Hopefully this helps, Jen Galaxy team -- Jennifer Jackson http://galaxyproject.org
ADD COMMENTlink written 6.3 years ago by Jennifer Hillman Jackson25k
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