Question: gff to gtf conversion
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computationalvarun • 20 wrote:
hi i have uploaded gff format GRCh37.p13 in glaxy. however i am unable to see it on doing any analysis like for tophat and cufflink. how can i do it. how do i convert it to gtf for using it as a reference genome for tophat. it is a human genome. thanks a lot. regards
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modified 18 months ago
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Guy Reeves • 1.0k
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2.0 years ago by
computationalvarun • 20
Is it really a GFF file or does Galaxy just think it is (Galaxy will label GTF files as GFF when you upload them unless you explicitly tell it that the file has GTF format)? If it's really a GTF file then just upload it again and explicitly select "GTF" as the file type.
thank you devon. i will try again. in tophat using full parameter list i am choosing use gene annotation model. in that it is not showing grch37 although it is showing it in my history of galaxy account. i hav echosen hg19 as reference genome. how to go about it. thanks