Question: Gene Or Transcript Differential Expression Testing
gravatar for i b
6.3 years ago by
i b330
i b330 wrote:
Hi, I am a bit confused about two outputs of cuffdiff: If I want to see if two samples, e.g. treated and un-treated express similar protein levels, what would be best to look at gene differential expression or transcript diff. expression? Thanks, ib
rna-seq cuffdiff • 1.2k views
ADD COMMENTlink modified 6.3 years ago by Leonor Palmeira10 • written 6.3 years ago by i b330
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6.3 years ago by
Leonor Palmeira10 wrote:
Hash: SHA1 Hi, proteins are synthetised from transcripts, not from genes, so I wouldn't use gene DE. It can become quite tricky to interpret these for some alternative transcripts. Beast, Leonor. - -- Leonor Palmeira, PhD Phone: +32 4 366 42 69 Email: mlpalmeira AT ulg DOT ac DOT be Immunology-Vaccinology, Bat. B43b Faculty of Veterinary Medicine Boulevard de Colonster, 20 University of Liege, B-4000 Liege (Sart-Tilman) Belgium Version: GnuPG v1.4.10 (GNU/Linux) Comment: Using GnuPG with Mozilla - iQEcBAEBAgAGBQJQFBCdAAoJEKquFGwgRb3zchgH/3bGHlV4igMxp3eDhUCRPK5m Fa1I5Y7fvlSZXCPVjiwpoO1j6uXSiYnNYxGTPWYet4qaTKHee0lRtfPLbXhpC4t+ BgEh8HaCoruUStM9qOG/d0ljWjEwM0QRdw1/oWVvF1mLwYq+LBIZdAw0yR/9bTYo xvdmd7Qd+bDq/vh8vR4gn/Cr6oOoB16eMz++X+DXk7pUuQXXxuACt7TEU4tf5Isz ZzlpjVt0a5Y1tYuc5MA4zuhDZzB6u3NEAMzufz3VDgVx/QCX7hKQkSi6rYBaRAmu EdPnLwvVQhOcqaSGz9wSdkkeTHVcE5gmkJ8ZV6re3jua4iI9vW9Lf4ifPQCXamU= =9Frm
ADD COMMENTlink written 6.3 years ago by Leonor Palmeira10
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