Question: Questions On Directionality
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Maness, Nicholas J • 10 wrote:
Hi there,
I apologize if this is covered in documentation or help threads.
searched and it seemed it was not. I have several illumina rna-seq
data sets that "should" be directional. It seems the directionality
is very good, based on the visualization. First question is; in the
visualization window, are the reads color coded by direction, i.e. are
orange one direction and blue the other? If so, it looks like my data
is >95% directional. Similar question, is there a way to quantify
directionality of the data set?
BTW, the data is RNA-seq data from rhesus macaques and i'm using the
rhesus macaque genome build from UCSC.
thanks very much,
Nick Maness
Tulane University
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modified 6.8 years ago
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Jeremy Goecks • 2.2k
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6.8 years ago by
Maness, Nicholas J • 10