Question: bed file to bigwig file conversion
0
gravatar for leila.hosseinzadeh
4 weeks ago by
leila.hosseinzadeh0 wrote:

Hello there,

I want to convert my bed files to bigwig files in order to visualize them using IGV. Is there any way to convert them using GALAXY? if not, can you please help me with this?

Thank you in advance.

igv bigwig bedgraph chip-seq • 100 views
ADD COMMENTlink modified 4 weeks ago • written 4 weeks ago by leila.hosseinzadeh0
0
gravatar for Jennifer Hillman Jackson
4 weeks ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

If you already have bed data that includes a score value (4th column), then use Cut to reduce the data to just the first four columns (as needed) to create a bedgraph dataset, then assign that datatype. The result can be converted to bigWig format with the tool: Wig/BedGraph-to-bigWig converter.

FAQ: https://galaxyproject.org/support/

Thanks, Jen, Galaxy team

ADD COMMENTlink written 4 weeks ago by Jennifer Hillman Jackson25k
0
gravatar for leila.hosseinzadeh
4 weeks ago by
leila.hosseinzadeh0 wrote:

I've got the bigwig files, Thank you so much.

ADD COMMENTlink written 4 weeks ago by leila.hosseinzadeh0
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