Hi, I'am new in NGS and not very familiar with data analysis. I'am wondering how can i proceed with data from targeted DNA with UMIs? there is any workflow in Galaxy? What does that mean: 2 read pairs: that does mean paired end sequencing or 2 cycles paired end sequencing? Thank you in advance
Galaxy tutorials can be found here: https://galaxyproject.org/learn/
There are also public Galaxy Domain-specific servers that include specialized tools/workflows, please see: https://galaxyproject.org/public-galaxy-servers/
When a tool form states something similar to "read pairs" for an input, this nearly always means paired-end reads (two inputs, unless the data is already in a paired collection). Which tool are you looking at if this does not clarify the usage?
Thanks! Jen, Galaxy team
Hello Jen, Thank you for your answers. When i got my fastq, adaptor sequences are already trimmed? and data demultiplexed? i have to: upload data on Galxy QC Trimming adaptor, umi traitement? or already trimmed before you got the Fastq? Mapping indel call ... Is that ok for you? Thank you