Hello everyone, I'm very new in RNA seq analysis I have run cuffdiff on 3 condition dataset collections( control and 2 different treatment groups) using GALAXY, then I got an error, I have repeated the same job many times, unfortunately, I got the same result.
Fatal error: Exit code 1 () [04:06:22] Loading reference annotation. [04:06:23] Inspecting maps and determining fragment length distributions. [04:30:24] Modeling fragment count overdispersion. [04:32:50] Modeling fragment count overdispersion. [04:35:14
I also tried to use annotation file (GTF) of the same genome build I have used in cufflink instead of cuffmerge transcriptome GTF file but got the same problem
Any help will be appreciated. Regards
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