I want to assemble a transcriptome with Trinity using reads that are both paired- and single-end. Due to memory issues I have to normalize the reads, but the normalization process in Trinity is not able to handle this kind of data. Is there any application in Galaxy that I can use to normalize the reads prior to the assembly process? Data is in fastqsanger format
Thank you very much for your time.
error from the randomization step: Error, pairs.K25.stats is empty. Be sure to check your fastq reads and ensure that the read names are identical except for the /1 or /2 designation. at /opt/packages/trinity/2.2.0/util/insilico_read_normalization.pl line 882.