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Question: Cufflinks Fpkm
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gravatar for Li, Jilong (MU-Student)
7.1 years ago by
Li, Jilong (MU-Student) • 80
Li, Jilong (MU-Student) • 80 wrote:
Hi, I want to use cufflinks handle the results of Tophat. Cufflinks uses FPKM to normalize the expression data. I think FPKM is for pair-end reads. right? My reads are single-end. Is it right if I use FPKM? Thank you very much! Victor
rna-seq cufflinks • 1.3k views
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modified 6.9 years ago by Jennifer Hillman Jackson ♦ 25k • written 7.1 years ago by Li, Jilong (MU-Student) • 80
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gravatar for Jennifer Hillman Jackson
6.9 years ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:
Hello Victor, This is OK for use with single-end reads. But, if you have questions about how to exactly interpret the values, the Cufflinks authors would be a good resource, the contact information is in the example of this wiki Support section: http://wiki.g2.bx.psu.edu/Support#Unexpected_scientific_result Hopefully this helps, Best, Jen Galaxy team -- Jennifer Jackson http://usegalaxy.org http://galaxyproject.org/wiki/Support
ADD COMMENT • link written 6.9 years ago by Jennifer Hillman Jackson ♦ 25k
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