I'm trying to run a DESeq2 analysis of my ten RNASeq Samples. I successfully was able to use the htseq analysis for these, generating one file with counts and one file listed as (no feature) for each of the 10 samples. From there I've been trying in vain to do DESeq2.
I name the factor and have tried setting Factor 1 as Control and Factor 2 as Treatment and viceversa. I keep everything as default save normalizing the outliers, and select all my treatment and control groups for analysis including the no feature groups. I keep receiving a data set error, where the Factor 2 data sets regardless of being control or treatment seem to have a problem.
An error occurred with this dataset: DESeq2 run information
Any ideas? Thanks in advance!