Question: Total genomic DNA Concentration for cows (healthy and infected) milk microbiome study
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nazmul.hoque90 • 0 wrote:
I have extracted DNA from cows milk using Promega FFS Nucleic Acid extraction kits and Promega blood cell DNA purification kits in Maxwell 16 automated system. Now after quantification and bio-analysis, I performed NGS following library preparation using Illumina Nextera XT library preparation kits (NextSeq 500 machine) with a concentration of 0.2ng DNA per micro-liter, and got the sequence result. After QC and downstream analyses, I found only 0.122 million mapped reads/sample, which is too low for a typical microbiome study. I need at least 4.0 - 6.0 million mapped reads/sample from the DNA samples. What can I do now? or Shall I go for further DNA concentration? If so, how I can concentrate the DNA samples?
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modified 8 months ago
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Jennifer Hillman Jackson ♦ 25k
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8 months ago by
nazmul.hoque90 • 0
