Question: FASTQC for RNAseq
0
gravatar for ramibalasubramanian
4 months ago by
ramibalasubramanian0 wrote:

i'm new to this RNAseq data analysis and im a learner. i took the RNA-seq of dengue virus when i checked for quality control my gc% was 47% and failed. i donno how to process this data. if anyone can help please suggest me some solutions

fastq gc fastqc qa rna-seq • 171 views
ADD COMMENTlink modified 4 months ago by Jennifer Hillman Jackson25k • written 4 months ago by ramibalasubramanian0
0
gravatar for Jennifer Hillman Jackson
4 months ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

Please see this Galaxy tutorial for how to perform QA/QC: https://galaxyproject.org/tutorials/ngs/

A failed GC content result from FastQC does not necessarily mean that the data is unusable. Details: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/3%20Analysis%20Modules/5%20Per%20Sequence%20GC%20Content.html

Thanks! Jen, Galaxy team

ADD COMMENTlink written 4 months ago by Jennifer Hillman Jackson25k
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