Question: FreeBayes returning an empty table
0
gravatar for annet.kyomuhangi
6 months ago by
annet.kyomuhangi10 wrote:

Hi there, If I provide Freebayes with quality scores it gives me an empty table. If I don't provide the quality scores it returns a full table. What could be wrong?

Thank you

galaxy variant vcf bam freebayes • 157 views
ADD COMMENTlink modified 6 months ago by Jennifer Hillman Jackson25k • written 6 months ago by annet.kyomuhangi10
0
gravatar for Jennifer Hillman Jackson
6 months ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

The input for FreeBayes is a mapped BAM dataset. The input to the mapping tool in used upstream (Bowtie2, BWA/MEM) would contain quality scores, even if provided as default values (using a tool like Combine FASTA and QUAL into FASTQ). Perhaps the jobs were not run with a mapped BAM input?

At Galaxy Main https://usegalaxy.org, I see at least one successful FreeBayes job in your recent histories. It uses proper inputs and produces the correct output. I wasn't able to find the jobs with this type of input/output.

If you still need help, and are working at Galaxy main, an email can be sent to galaxy-bugs@lists.galaxyproject.org from your registered account. Include details about the exact history (share link to history) and FreeBayes output dataset numbers that represent this issue. Make sure all inputs/outputs are left undeleted for full review/feedback. Undelete (if just deleted) or reproduce (if permanently deleted). If there is a problem, we'd like to clarify and fix it.

Galaxy tutorials, including those for Variant Analysis: https://galaxyproject.org/learn/

Thanks!

ADD COMMENTlink modified 6 months ago • written 6 months ago by Jennifer Hillman Jackson25k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 99 users visited in the last hour