ChIP seq files sequenced in Sanger / illumina 1.9, when processed for mapping with Bowtie for Illumina, showed the following error: "Required metadata values are missing. Some of these values may not be editable by the user. Selecting "Auto-detect" will attempt to fix these values."
Further info said: "Error aligning sequence. Error: reads file does not look like a FASTQ file terminate called after throwing an instance of 'int' /bin/sh: line 1: 9127 Aborted (core dumped) bowtie -q -p 6 -S -n 2 -e 70 -l 28 --maxbts 125 -k 1 -m 1 --phred" Why is it showing this?
While uploading the file through FTP, i had by mistake selected the format type to be Fastq.gz. But later i went to the file attributes and changed the data type to fastqsanger. Is there something to do with this?
For the time being, Metadata is in auto detection mode. But it has been like that for the past 1 hr. What should i do?