Question: Problem in cuffdiff
14 months ago by
sinha.puja • 0
sinha.puja • 0 wrote:
Hi everyone...after doing cuffdiff when viewing the data in tabular format of gene differential expression testing I am finding no fold change values, no test statistics, p and q values are 1 and there is no significant found in all over the table...so how this is possible...explain...or I think I have done something wrong but where...at one step I have a doubt that before doing tophat, the column has options like paired end sequencing or single...mine paired end but after that there is options like forward reads and reverse reads...I have placed forward reads and reverse reads separately so do I need to pair forward and reverse reads before putting in the option..???
ADD COMMENT • link •modified 14 months ago by Jennifer Hillman Jackson ♦ 25k • written 14 months ago by sinha.puja • 0